Spino-diencephalic relays through the parabrachial nucleus in the cat

Previous studies have shown that the spinal input to the parabrachial nucleus (PBN) in the cat is limited to certain portions of its lateral division 8,21,45. The purpose of the present study was to determine some of the output targets of PBN neurons located within this spinal terminal domain by means of single, double and triple light microscopic labeling strategies. Combinations of tracers included the retrograde transport of tritiated wheat germ agglutinin, wheat germ agglutinin conjugated with horseradish peroxidase (WGA-HRP) and Fluoro-Gold from the hypothalamus, amygdala or thalamus/zona incerta together with either the anterograde transport of WGA-HRP from the spinal cord or the degeneration of spinal terminals following spinal lesions. The results (summarized in Fig. 10) showed that the spinal terminal domain contains separable populations of neurons projecting to the thalamus/zona incerta and hypothalamus. Only a limited number of amygdala-projecting neurons was located in this domain. Evidence from several laboratories supports the conclusion that these potential spino-diencephalic relays are involved somehow in nociception. More information is needed, however, regarding differences in the response properties of these separable populations of spinal-recipient neurons before more specific hypotheses concerning the precise nature of their nociceptive functions can be formulated.