Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

7.7K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
7.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A bistable catanionic system with reinforced thermal stability.

Journal of colloid and interface science·2026
Same author

Allosteric coupling between a lipid bilayer and a membrane protein.

Biophysical journal·2025
Same author

Fine-tuning the yeast GAL10 promoter and growth conditions for efficient recombinant membrane protein production and purification.

Protein science : a publication of the Protein Society·2025
Same author

Molecular and Supramolecular Study of Uranium/Plutonium Liquid-Liquid Extraction with <i>N</i>,<i>N</i>-Dialkylamides.

Inorganic chemistry·2024
Same author

Hydrophobic Porous Liquids with Controlled Cavity Size and Physico-Chemical Properties.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2023
Same author

Influence of Hydrophobic Groups Attached to Amphipathic Polymers on the Solubilization of Membrane Proteins along with Their Lipids.

Analytical chemistry·2022

Related Experiment Video

Updated: May 1, 2026

Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water
09:43

Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water

Published on: February 20, 2013

11.5K

Labeling and functionalizing amphipols for biological applications.

Christel Le Bon1, Jean-Luc Popot, Fabrice Giusti

  • 1Laboratoire de Biologie Physico-Chimique des Protéines Membranaires, UMR 7099, Institut de Biologie Physico-Chimique (FRC 550), CNRS/Université Paris 7, 13 rue Pierre et Marie Curie, 75005, Paris, France.

The Journal of Membrane Biology
|April 4, 2014
PubMed
Summary
This summary is machine-generated.

Amphipols (APols) are stable alternatives to detergents for membrane protein studies. This review details APol derivatives and their synthesis, highlighting advantages for biophysical research.

More Related Videos

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

14.6K
Investigating Single Molecule Adhesion by Atomic Force Spectroscopy
09:48

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Published on: February 27, 2015

9.7K

Related Experiment Videos

Last Updated: May 1, 2026

Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water
09:43

Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water

Published on: February 20, 2013

11.5K
Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

14.6K
Investigating Single Molecule Adhesion by Atomic Force Spectroscopy
09:48

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Published on: February 27, 2015

9.7K

Area of Science:

  • Biochemistry
  • Biophysics
  • Polymer Chemistry

Background:

  • Membrane proteins (MPs) require stabilization in aqueous solutions, often using detergents.
  • Amphipols (APols) offer superior stability for MPs compared to traditional detergents.
  • A8-35 is a well-characterized APol extensively used in MP research.

Purpose of the Study:

  • To review APol derivatives developed in the laboratory.
  • To discuss the synthesis, labeling, and functionalization of APols.
  • To evaluate the advantages and disadvantages of various synthetic routes for APols.

Main Methods:

  • Development of various APol derivatives based on A8-35.
  • Exploration of labeling and functionalization strategies for APols.
  • Analysis of synthetic routes for APol production and purification.

Main Results:

  • APols enhance the stability of membrane proteins in aqueous solutions.
  • Labeled APols facilitate diverse studies including biodistribution, MP association, and complex dynamics.
  • Multiple functionalization of MP/APol complexes is achievable by mixing modified APols.

Conclusions:

  • APols are versatile tools for membrane protein research, offering enhanced stability and labeling capabilities.
  • The review provides insights into the synthesis and application of various APol derivatives.
  • Understanding synthetic routes is crucial for optimizing APol utility in biochemical and biophysical studies.