Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A versatile phage lambda expression vector system for cloning in Escherichia coli.

K Sieg1, J Kun, I Pohl

  • 1Institut für Genetik der Universität zu Köln, F.R.G.

Gene
|February 20, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Polarization splitting with cubic crystals evaluated with synchrotron radiation.

The Review of scientific instruments·2021
Same author

Extension of single-crystal x-ray spectropolarimetry with cubic crystals beyond perfect polarization-splitting geometries.

The Review of scientific instruments·2021
Same author

Cubic crystals in an x-ray polarization-splitting geometry.

The Review of scientific instruments·2020
Same author

Upregulation of extraneuronal TRPV1 expression in chronic rhinosinusitis with nasal polyps.

Rhinology·2018
Same author

TRPA1 receptor is upregulated in human oral lichen planus.

Oral diseases·2016
Same author

Orientation relationship of eutectoid FeAl and FeAl<sub>2</sub>.

Journal of applied crystallography·2016

Researchers developed new phage expression vectors, lambda JK2 and lambda JK4, for efficient DNA cloning and fusion protein expression. This facilitates easy analysis of recombinant clones, including Plasmodium falciparum DNA, for potential antigen discovery.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Bacteriophage lambda vectors are crucial for gene cloning and expression.
  • Fusion proteins offer a means to study gene function and protein interactions.
  • Efficient screening of recombinant clones is essential for library construction.

Purpose of the Study:

  • To construct novel phage expression vectors, lambda JK2 and lambda JK4.
  • To enable efficient cloning of genomic or cDNA into the lacZ gene.
  • To facilitate the expression and analysis of fusion proteins.

Main Methods:

  • Integration of expression plasmids pJK2 and pJK4 into bacteriophage lambda.
  • Cloning of genomic or cDNA into the 5' or 3' end of the lacZ gene.
  • Construction of a Plasmodium falciparum DNA expression library in lambda JK2.

Related Experiment Videos

Main Results:

  • Developed lambda JK2 and lambda JK4 phage expression vectors.
  • Demonstrated expression of fusion proteins with active beta-galactosidase (beta Gal).
  • Successfully constructed and screened a Plasmodium falciparum genomic DNA expression library.

Conclusions:

  • Lambda JK2 and lambda JK4 vectors allow efficient gene cloning and fusion protein expression.
  • Recombinant clone analysis is simplified by reversible plasmid integration.
  • Chimeric proteins can be affinity-purified and cleaved, enabling further characterization.