Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Micelles01:30

Micelles

364
Micelle formation is an intricate process that hinges on the properties of amphiphilic or amphipathic molecules and the conditions of the system in which they are found. Amphiphilic molecules, which have both hydrophilic (water-attracting) and hydrophobic (water-repelling) parts, play a critical role in this process.In aqueous environments, these molecules arrange themselves such that their hydrophilic heads are turned towards the water phase, while their hydrophobic tails are oriented away...
364
Detergent Purification of Membrane Proteins01:18

Detergent Purification of Membrane Proteins

5.5K
Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
5.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intrinsic Structure of Lipoplexes Embedded in Polyelectrolyte Multilayers.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Pulmonary delivery of anti-infectives from natural sources: Development and characterization of liposomal sanggenon formulations.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences·2025
Same author

Daptomycin membrane activity is modulated by the localized interplay between calcium ions and phospholipids in monolayers and bilayers containing a lysyl-phosphatidylglycerol analogue.

Biochimica et biophysica acta. Biomembranes·2025
Same author

A Deployable 4D Printed, Mucoadhesive and Magnetically Guided Patch for Local Therapy of Gastric Cancer.

Advanced healthcare materials·2025
Same author

The 8th International Symposium on Phospholipids in Pharmaceutical Research - An update on current research in phospholipids presented at the biennial symposium of the Phospholipid Research Center Heidelberg.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences·2025
Same author

Editorial to 'Bio-material interfaces and Drug Delivery'.

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V·2025
Same journal

Laser-Assisted Electrochemical Deposition of Bilateral Au Coatings on Ni Foils: Mechanism and Experimental Study.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Mechanistic Insights into Pulmonary Surfactant Inactivation.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

MPN-GE Bilayer Interphase Construction: Green Modification Derived from Biomass and Synergistic Enhancement of CFRP.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Magnetically Retrievable Core@Shell Nanocomposites for Rare Earth Element Adsorption: Experimental and Machine Learning Insights.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Microstreaming of a Pneumatically Controlled Bubble under Hydrostatic Pressure and Crossflow.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Tuning Pore Sizes of Core-Shell Dendritic Mesoporous Silica Nanoparticles for Efficient Loading of Functional Materials.

Langmuir : the ACS journal of surfaces and colloids·2026
See all related articles

Related Experiment Video

Updated: May 1, 2026

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
08:29

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

9.3K

New micellar transfection agents.

Christian Wölk1, Dorota Pawlowska, Simon Drescher

  • 1Institute of Pharmacy, Martin-Luther-Universitaet (MLU) Halle-Wittenberg , Wolfgang-Langenbeck-Strasse 4, 06120 Halle (Saale), Germany.

Langmuir : the ACS Journal of Surfaces and Colloids
|April 5, 2014
PubMed
Summary
This summary is machine-generated.

Novel amino-functionalized lipids, OT6 and TT6, form stable micelles for effective DNA complexation. These lipid-DNA complexes show promise as polynucleotide delivery agents due to their colloidal stability and reproducible micelle production.

More Related Videos

A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles
09:57

A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles

Published on: December 23, 2016

8.3K
Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation
07:40

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation

Published on: July 31, 2015

8.0K

Related Experiment Videos

Last Updated: May 1, 2026

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
08:29

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

9.3K
A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles
09:57

A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles

Published on: December 23, 2016

8.3K
Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation
07:40

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation

Published on: July 31, 2015

8.0K

Area of Science:

  • Biochemistry
  • Materials Science
  • Nanotechnology

Background:

  • Amino-functionalized lipids are crucial for developing effective drug and gene delivery systems.
  • Developing novel lipidic materials with controlled self-assembly properties is essential for advanced nanomedicine.
  • The production of stable micelle dispersions is a key challenge in lipid-based delivery systems.

Purpose of the Study:

  • To characterize two novel micelle-forming amino-functionalized lipids, OT6 and TT6.
  • To evaluate their potential as polynucleotide transferring agents.
  • To investigate the formation, stability, and DNA complexation of their micelle dispersions.

Main Methods:

  • Characterization of critical micelle concentration (cmc) using isothermal titration calorimetry (ITC) and 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescence.
  • Thermal analysis of lipid dispersions via differential scanning calorimetry (DSC), dynamic light scattering (DLS), Fourier-transform infrared (FT-FTIR) spectroscopy, and cryo-transmission electron microscopy (cryo-TEM).
  • Investigation of DNA complexation using ITC, DSC, DLS, ζ-potential measurements, X-ray scattering, and transmission electron microscopy (TEM).

Main Results:

  • OT6 and TT6 lipids successfully form stable micelle dispersions with reproducible characteristics.
  • The critical micelle concentration (cmc) for both lipids was determined using ITC and DPH fluorescence.
  • Effective complexation of DNA by OT6 and TT6 micelles was confirmed, forming stable lipoplexes with desirable colloidal properties.

Conclusions:

  • OT6 and TT6 represent promising novel materials for polynucleotide delivery.
  • The characterized micelle dispersions offer an advantageous and reproducible method for preparing lipid-DNA complexes.
  • The study demonstrates the potential of these lipids in forming stable, functional lipoplexes for gene transfer applications.