[Use of a peroxidase marker to detect the diffusion of protein in gel]

The method for the assessment of the protein gel diffusion rate implies the employment of protein conjugates with horseradish peroxidase; the diffusion of these conjugates can be easily visualized by covering the gel with a cellulose acetate film wetted with a solution of the substrate specific for peroxidase. The necessary concentration of the protein can be selected with this method, as well as the time of the diffusion of the proteins tested as chemoattractants in studies on cellular chemotaxis under an agar layer.