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Updated: May 1, 2026

Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis
Published on: September 28, 2022
Highly efficient multiplex targeted mutagenesis and genomic structure variation in Bombyx mori cells using
Yuanyuan Liu1, Sanyuan Ma1, Xiaogang Wang1
1State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, PR China.
Abstract:
Bombyx mori is an economically important insect and a model organism for studying lepidopteran and arthropod biology. Using a highly efficient CRISPR/Cas9 system, we showed that this system could mediate highly efficient targeted genome editing of a single gene locus, large chromosomal deletion or inversion, and also multiplex genome editing of 6 genes simultaneously in BmNs cell line derived from B. mori. The simplicity and high efficiency of our system provide unprecedented possibilities for researchers to implement precise and sophisticated manipulation of a chosen B. mori gene in BmNs cells easily in a limited time course, and perhaps new opportunities for functional genomics of B. mori and other lepidopteran insects.
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