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Effect of IL-2 on suppressor factor production.
J Freire-Moar1, J A Kapp, D R Webb
1Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110.
Lymphokine Research
|January 1, 1989
Summary
Interleukin-2 (IL-2) can stimulate suppressor T-cells to produce antigen-specific suppressor factors (TsF2). This occurs even though IL-2 does not affect their growth, suggesting a novel signaling role for IL-2 in immune regulation.
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- Suppressor T-cell hybridomas specific for the GAT antigen release suppressor factors (TsF) spontaneously.
- These hybridomas do not require exogenous lymphokines for growth, and IL-2 has no effect on their proliferation or protein biosynthesis.
Purpose of the Study:
- To investigate the effect of IL-2 on suppressor T-cell hybridomas.
- To explore the role of IL-2 in the production of antigen-specific suppressor factors (TsF2).
Main Methods:
- Utilized suppressor T-cell hybridomas specific for the GAT antigen.
- Assessed TsF2 production via bioactivity and 35S-methionine incorporation.
- Investigated the impact of phorbol myristate acetate (PMA) on IL-2 receptor expression and TsF2 biosynthesis.
Main Results:
- IL-2 significantly increased TsF2 production in the 762 B3.7 hybridoma, despite not affecting cell growth.
- PMA treatment enhanced IL-2 receptor expression and augmented IL-2's effect on TsF2 biosynthesis.
- Data indicate IL-2's role in triggering suppressor cells to produce TsF2.
Conclusions:
- IL-2 may act as a signaling molecule to induce suppressor T-cells to produce antigen-specific suppressor factors.
- This function of IL-2 is distinct from its known growth-promoting properties.
- Findings suggest a novel mechanism for immune regulation involving IL-2 and suppressor T-cells.