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Updated: May 1, 2026
Reconstruction of 3-Dimensional Histology Volume and its Application to Study Mouse Mammary Glands
Published on: July 26, 2014
Registration of in vivo prostate MRI and pseudo-whole mount histology using Local Affine Transformations guided by
Chaitanya Kalavagunta1, Xiangmin Zhou, Stephen C Schmechel
1Center of Magnetic Resonance Research, University of Minnesota, Minneapolis, Minnesota, USA.
Purpose:
To present a novel registration approach called LATIS (Local Affine Transformation guided by Internal Structures) for coregistering post prostatectomy pseudo-whole mount (PWM) pathological sections with in vivo MRI (magnetic resonance imaging) images.
Materials And Methods:
Thirty-five patients with biopsy-proven prostate cancer were imaged at 3T with an endorectal coil. Excised prostate specimens underwent quarter mount step-section pathologic processing, digitization, annotation, and assembly into a PWM. Manually annotated macro-structures on both pathology and MRI were used to assist registration using a relaxed local affine transformation approximation. Registration accuracy was assessed by calculation of the Dice similarity coefficient (DSC) between transformed and target capsule masks and least-square distance between transformed and target landmark positions.
Results:
LATIS registration resulted in a DSC value of 0.991 ± 0.004 and registration accuracy of 1.54 ± 0.64 mm based on identified landmarks common to both datasets. Image registration performed without the use of internal structures led to an 87% increase in landmark-based registration error. Derived transformation matrices were used to map regions of pathologically defined disease to MRI.
Conclusion:
LATIS was used to successfully coregister digital pathology with in vivo MRI to facilitate improved correlative studies between pathologically identified features of prostate cancer and multiparametric MRI.
