A new method for yeast phagocytosis analysis by flow cytometry
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Enzymatic Modification and Flow Cytometry Assessment of Yeast Surface Displayed Proteins
Published on: May 30, 2025
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Catarina Carneiro1, Catarina Vaz1, Joana Carvalho-Pereira1
1Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal.
Journal of Microbiological Methods
|April 8, 2014
Abstract:
Herein we developed a method based on the quenching effect of propidium iodide over Sytox-Green fluorescence to assess yeast phagocytosis by flow cytometry. It allows accurate quantification of living from dead phagocytes; internalized from non-internalized cells, maintaining yeast fluorescence within phagocytes; and the different associations between phagocytes and fungal cells.

