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In vitro Reconstitution of the Active T. castaneum Telomerase
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In vitro Reconstitution of the Active T. castaneum Telomerase

Published on: July 14, 2011

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Telomerase structure.

Sara Sandin1, Daniela Rhodes2

  • 1School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.

Current Opinion in Structural Biology
|April 8, 2014
PubMed
Summary
This summary is machine-generated.

New research reveals the 3D structure of human and ciliate telomerase, showing human telomerase is dimeric and functional only as a dimer, unlike its monomeric ciliate counterpart. This highlights evolutionary differences in telomerase composition and function.

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Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
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Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • Telomerase reverse transcriptase is crucial for telomere maintenance and cancer development.
  • Recent advancements have enabled high-resolution structural determination of telomerase holoenzyme complexes.

Purpose of the Study:

  • To elucidate the three-dimensional architecture of human and ciliate telomerase.
  • To compare the structural and compositional differences between human and ciliate telomerase.

Main Methods:

  • Single particle electron microscopy (EM) was used to obtain ~25Å resolution structures.
  • Protein and RNA subunit locations were assigned within the EM density maps.
  • Crystal structures of the catalytic protein subunit were fitted into the maps.

Main Results:

  • Human telomerase forms a functional dimer, while ciliate telomerase is monomeric.
  • Both structures share a common RNA/reverse transcriptase core.
  • Significant differences in RNA size and protein composition were observed, suggesting evolutionary divergence.

Conclusions:

  • The dimeric state of human telomerase is essential for its function.
  • Evolution has led to distinct oligomeric states and subunit compositions in telomerase across different organisms.
  • Structural insights provide a basis for understanding telomerase function in health and disease.