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Related Experiment Video

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Creating and Applying a Reference to Facilitate the Discussion and Classification of Proteins in a Diverse Group

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FixPred: a resource for correction of erroneous protein sequences.

Alinda Nagy¹, László Patthy

¹Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, H-1113 Budapest, Hungary.

Database : the Journal of Biological Databases and Curation

|April 8, 2014

Summary

Protein databases contain errors that affect analyses. The FixPred pipeline automatically corrects these erroneous sequences, improving data reliability for multiple species.

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Area of Science:

Bioinformatics
Genomics
Proteomics

Background:

Protein sequence databases are prone to errors, including mispredictions, abnormalities, and incompleteness.
These erroneous sequences can significantly distort findings from genome-scale protein analysis.

Purpose of the Study:

To present the FixPred computational pipeline for automatically correcting erroneous protein sequences.
To provide an updated FixPred database with corrected sequences from key species.

Main Methods:

Utilized the MisPred resource to identify erroneous sequences.
Developed the FixPred computational pipeline to automatically correct identified erroneous sequences.
Curated UniProtKB/Swiss-Prot and NCBI/RefSeq databases for multiple species.

Main Results:

The FixPred pipeline successfully corrects erroneous protein sequences.
The FixPred database now includes corrected sequences for Homo sapiens, Mus musculus, Rattus norvegicus, and other Metazoan species.
A web interface, query engine, and web service facilitate access and data retrieval.

Conclusions:

The FixPred pipeline and database offer a valuable resource for improving the accuracy of protein sequence data.
Automated correction of erroneous sequences enhances the reliability of genomic and proteomic analyses.
Future releases will expand the database to include more Metazoan species.