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Long non-coding RNA 91H contributes to the occurrence and progression of esophageal squamous cell carcinoma by

Tianyi Gao1, Bangshun He, Yuqin Pan

  • 1Central Laboratory, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.

Molecular Carcinogenesis
|April 8, 2014
PubMed

Insights

Long non-coding RNA 91H acts as a tumor suppressor in esophageal squamous cell carcinoma (ESCC). Lower 91H expression correlates with advanced ESCC, suggesting its role in regulating IGF2 expression and disease progression.

Area of Science:

  • Molecular Biology
  • Oncology
  • Epigenetics

Background:

  • Long non-coding RNAs (lncRNAs) are key regulators in biological systems.
  • The lncRNA 91H, located at the H19/IGF2 locus, is implicated in tumor suppression.
  • Its specific role in esophageal squamous cell carcinoma (ESCC) remains largely unelucidated.

Purpose of the Study:

  • To investigate the function of lncRNA 91H in regulating H19 imprinting control regions (ICR) and IGF2 expression.
  • To determine the association between 91H expression and ESCC progression.
  • To explore the potential of 91H as a biomarker for ESCC risk and prognosis.

Main Methods:

  • Analysis of 91H expression in ESCC cell lines (TE-1, Eca-109) and 232 patient tumor samples.
  • Treatment of cell lines with a demethylation agent to assess the effect on 91H expression.
  • Knockdown of 91H in cell lines to evaluate its impact on IGF2 expression.
  • Correlation analysis between 91H expression and clinicopathological features (depth of invasion, grading, TNM stage).

Main Results:

  • 91H expression was significantly lower in ESCC patients with advanced disease (higher depth of invasion, neoplastic grading, TNM stage).
  • 91H expression decreased in ESCC cell lines upon treatment with a demethylation agent.
  • Knockdown of 91H led to a significant increase in IGF2 expression.
  • Lower 91H expression was associated with overexpression of IGF2, indicating its inhibitory role.

Conclusions:

  • lncRNA 91H is epigenetically regulated and inhibits IGF2 expression in ESCC.
  • Reduced 91H expression is linked to ESCC progression and poor prognosis.
  • 91H may serve as a novel biomarker for identifying patients at increased risk for ESCC occurrence, progression, and adverse outcomes.
Keywords:
91HESCCH19 ICRIGF2expressionlncRNAmethylation

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