Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

3.1K
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
3.1K
Lineage Commitment01:21

Lineage Commitment

3.4K
Commitment is the  process whereby stem cells:
3.4K
Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

3.4K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Regulatory B cells contribute to allergen-encapsulating nanoparticle immunotherapy efficacy for food allergy.

JCI insight·2026
Same author

Myeloid Cell-Targeting PLGA Nanoparticles Ameliorate Acute Graft-Versus-Host Disease.

Cancers·2026
Same author

Comprehensive Multiplatform Tyrosine Kinase Profiling Reveals Novel Actionable FGFR Aberrations across Sarcomas Affecting the Young.

Molecular cancer therapeutics·2026
Same author

Antigen-specific immunotherapy with a CD4<sup>+</sup> T cell neoepitope restrains CD8<sup>+</sup> T cell differentiation in murine pancreatic islet grafts.

Nature communications·2026
Same author

Comprehensive multi-platform tyrosine kinase profiling reveals novel actionable FGFR aberrations across sarcomas affecting the young.

Molecular cancer therapeutics·2026
Same author

Losartan Alleviates Chemical Burn-Induced Limbal Stem Cell Deficiency: Repurposing a Venerable Anti-Hypertension Drug.

Investigative ophthalmology & visual science·2026
Same journal

Retraction notice to "Myeloid-derived suppressor cells accumulate in the liver site after sepsis to induce immunosuppression" Cellular Immunology 279 (2012) 12-20.

Cellular immunology·2026
Same journal

The uptake, intracellular trafficking and recycling of FcRn-blocking therapeutics in human endothelial cells in vitro.

Cellular immunology·2026
Same journal

Exosome-mediated immune modulation in rheumatoid arthritis and its role in synovial inflammation and autoimmune amplification.

Cellular immunology·2026
Same journal

Persistent CD4<sup>+</sup> T cell hyporesponsiveness during recovery from prolonged symptomatic SARS-CoV-2 infection.

Cellular immunology·2026
Same journal

Deficit in blood MAIT cells, altered cytokines and T cell dynamics in patients with coronary artery disease.

Cellular immunology·2026
Same journal

The circ_0003692-miR-1197-TLR4 axis: A key regulator in rheumatoid arthritis proliferation and inflammation.

Cellular immunology·2026
See all related articles

Related Experiment Video

Updated: May 1, 2026

Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages
09:57

Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages

Published on: August 12, 2019

11.5K

Molecular control of monocyte development.

Rachael L Terry1, Stephen D Miller1

  • 1Department of Microbiology-Immunology and Interdepartmental Immunobiology Center, Feinberg School of Medicine, Northwestern University, USA.

Cellular Immunology
|April 9, 2014
PubMed
Summary
This summary is machine-generated.

Monocyte development involves key factors, but molecular controls for Ly6C(hi) and Ly6C(lo) subsets remain unclear. This review summarizes transcriptional regulation and its role in microglial development.

Keywords:
IRF8KLF4Ly6C(hi) monocytesLy6C(lo) monocytesMicrogliaMonocyte developmentNR4A1PU.1Transcription factors

More Related Videos

Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags
09:32

Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags

Published on: September 9, 2014

72.5K
Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

9.3K

Related Experiment Videos

Last Updated: May 1, 2026

Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages
09:57

Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages

Published on: August 12, 2019

11.5K
Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags
09:32

Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags

Published on: September 9, 2014

72.5K
Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

9.3K

Area of Science:

  • Immunology
  • Cell Biology
  • Developmental Biology

Background:

  • Monocyte development is a complex, multi-staged process originating from hematopoietic progenitor cells.
  • Key transcription factors (e.g., PU.1, IRF8, KLF4) and growth factors (e.g., M-CSF, IL-34) are established regulators.
  • The precise molecular mechanisms governing differentiation into Ly6C(hi) inflammatory and Ly6C(lo) monocyte subsets require further elucidation.

Purpose of the Study:

  • To review the transcriptional regulation of monocyte development.
  • To discuss the role of these molecular controls in microglial development, despite differing origins.
  • To examine recent advances in understanding the differentiation of specific monocyte subpopulations.

Main Methods:

  • Literature review and synthesis of existing research on monocyte and microglial development.
  • Analysis of transcriptional regulators, growth factors, and cytokines involved in monocyte differentiation.
  • Examination of recent studies on mechanisms driving monocyte subpopulation differentiation.

Main Results:

  • Established knowledge on transcription factors, growth factors, and cytokines driving monocyte development from hematopoietic stem cells.
  • Identification of gaps in understanding the specific molecular controls for Ly6C(hi) and Ly6C(lo) monocyte subset differentiation.
  • Highlighting the conserved role of monocyte developmental regulators in microglial development.

Conclusions:

  • Transcriptional regulation is fundamental to monocyte development.
  • Further research is needed to fully define the molecular pathways differentiating monocyte subsets.
  • Understanding these pathways has implications for both immune cell function and neurodevelopmental processes.