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On electrophoretic NMR. Exploring high conductivity samples.

Michał Bielejewski1, Marianne Giesecke2, István Furó2

  • 1Division of Applied Physical Chemistry, Department of Chemistry, KTH Royal Institute of Technology, SE-10044 Stockholm, Sweden; Institute of Molecular Physics, Polish Academy of Sciences, M. Smoluchowskiego 17, 60-179 Poznań, Poland.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|April 9, 2014
PubMed
Summary

A new electrophoretic NMR (eNMR) method using a Carr-Purcell-Meiboom-Gill echo train with repeated electric field reversal (CPMGER) significantly reduces artifacts. This advance enables accurate electrophoretic mobility measurements without needing uncharged reference molecules.

Keywords:
CPMGDouble stimulated echoElectroosmosisSample cellThermal convectioneNMR

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Area of Science:

  • Analytical Chemistry
  • Physical Chemistry
  • Spectroscopy

Background:

  • Electrophoretic NMR (eNMR) is a powerful technique for determining molecular charge and size.
  • Convective flow artifacts, arising from electroosmotic and thermal forces, often limit the accuracy of eNMR measurements.
  • Existing eNMR methods typically require uncharged reference molecules to correct for these artifacts, which is not always feasible.

Purpose of the Study:

  • To investigate the performance of a novel eNMR pulse sequence designed to mitigate convective flow artifacts.
  • To demonstrate the effectiveness of this new method in various aqueous solutions.
  • To enable accurate electrophoretic mobility determination without the need for reference molecules.

Main Methods:

  • Implementation of a Carr-Purcell-Meiboom-Gill echo train with repeated electric field reversal (CPMGER).
  • Application of the CPMGER pulse sequence in eNMR experiments.
  • Analysis of aqueous solutions to assess artifact reduction and mobility accuracy.

Main Results:

  • The CPMGER pulse sequence significantly reduces artifacts caused by simultaneous electroosmotic and thermal driving forces.
  • Demonstrated improvements in the quality of eNMR spectra and accuracy of mobility measurements in various aqueous solutions.
  • Successful acquisition of electrophoretic mobilities without the use of uncharged reference molecules.

Conclusions:

  • The developed CPMGER pulse sequence is highly effective in suppressing convective flow artifacts in eNMR.
  • This method overcomes a significant limitation in eNMR, expanding its applicability to a wider range of samples and nuclei.
  • CPMGER eNMR offers a more robust and versatile approach for characterizing molecular electrophoretic properties.