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Related Experiment Video

Updated: May 1, 2026

Microcrystal Electron Diffraction of Small Molecules
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Microcrystal Electron Diffraction of Small Molecules

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Protein structure determination by MicroED.

Brent L Nannenga1, Tamir Gonen1

  • 1Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA.

Current Opinion in Structural Biology
|April 9, 2014
PubMed
Summary
This summary is machine-generated.

Microcrystal Electron Diffraction (MicroED) advances protein structure determination. This new method uses electron diffraction from tiny crystals to solve 3D protein structures, like lysozyme, offering a powerful crystallography approach.

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Area of Science:

  • Structural Biology
  • Biophysics
  • Crystallography

Background:

  • Determining protein structures is crucial for understanding biological function.
  • Traditional protein crystallography often requires large, high-quality crystals, which are difficult to obtain for many proteins.
  • Nanocrystals and microcrystals present challenges for conventional structure determination methods.

Purpose of the Study:

  • To review recent advances in structure determination from protein microcrystals.
  • To highlight the potential of Microcrystal Electron Diffraction (MicroED) as a novel technique.
  • To discuss the application of MicroED for solving 3D protein structures.

Main Methods:

  • Utilizing transmission electron cryo-microscopy to collect electron diffraction data.
  • Employing Microcrystal Electron Diffraction (MicroED) for structure determination.
  • Analyzing diffraction patterns from extremely small 3D protein crystals.

Main Results:

  • Demonstrated the capability of MicroED to solve the 3D structure of the model protein lysozyme.
  • Achieved a resolution of 2.9Å for the lysozyme structure using MicroED.
  • Showcased MicroED's effectiveness with nanocrystals.

Conclusions:

  • MicroED represents a significant advancement in protein crystallography.
  • The technique offers a unique and widely applicable approach for structure determination.
  • MicroED holds promise for broader application in structural biology as the method matures.