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Correlations of MUC15 overexpression with clinicopathological features and prognosis of glioma
1Department of Radiology, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
Abstract:
The abnormal expression of MUC15, a novel cell membrane-associated mucin, has been reported to predict poor survival in several cancers. The aim of the present study was to examine the expression of MUC15 in glioma and its correlation with clinicopathological features, including the survival of patients with glioma. The mRNA expression level of MUC15 was determined by RT-PCR, quantitative RT-PCR (RT-qPCR) and Western blotting in seven normal brain tissues and seven glioma tissues, respectively. The protein expression level of MUC15 was immunohistochemically detected in paraffin-embedded samples of 317 glioma tissues and 115 noncancerous brain tissues. The association of MUC15 expression levels with the clinicopathologic features and the prognosis was analyzed. The results showed that both mRNA and protein levels of MUC15 were significantly increased in glioma as compared with those in noncancerous brain tissue. Moreover, MUC15 overexpression was positively correlated with the advanced clinical stages of glioam patients (P<0.01). Furthermore, MUC15 expression levels were significantly correlated with the progression of glioma (P<0.001). Survival analysis indicated that glioma patients with higher MUC15 expression had a significantly shorter overall and 5-year survival time than those with low MUC15 expression. Multivariate analysis suggested that MUC15 overexpression was an independent factor for prognosis (hazard risk: 3.216; P=0.009). It was concluded that MUC15 is overexpressed in glioma tissues. Its overexpression correlates with tumor progression and it is a potentially unfavorable prognostic factor for patients with glioma.
Insights
Mucin 15 (MUC15) is overexpressed in glioma tissues, correlating with tumor progression. This MUC15 overexpression serves as an independent, unfavorable prognostic factor for glioma patients, indicating poorer survival outcomes.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Mucin 15 (MUC15), a cell membrane-associated mucin, exhibits abnormal expression linked to poor survival in various cancers.
- Understanding MUC15's role in glioma is crucial for developing new prognostic markers.
Purpose of the Study:
- To investigate MUC15 expression in glioma tissues.
- To analyze the correlation between MUC15 expression and clinicopathological features.
- To evaluate MUC15 as a prognostic factor for glioma patient survival.
Main Methods:
- Quantitative mRNA analysis using RT-PCR and RT-qPCR.
- Protein expression analysis via Western blotting and immunohistochemistry.
- Statistical analysis of MUC15 expression, clinicopathological data, and survival outcomes in 317 glioma and 115 noncancerous brain tissues.
Main Results:
- MUC15 mRNA and protein levels were significantly elevated in glioma tissues compared to noncancerous brain tissues.
- MUC15 overexpression positively correlated with advanced clinical stages and tumor progression in glioma.
- Higher MUC15 expression was associated with significantly shorter overall and 5-year survival times in glioma patients.
Conclusions:
- MUC15 is significantly overexpressed in glioma.
- MUC15 overexpression is a potential indicator of tumor progression and an unfavorable prognostic factor in glioma.
- MUC15 may serve as an independent prognostic biomarker for glioma patients.
