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Thermal Measurement Techniques in Analytical Microfluidic Devices
Published on: June 3, 2015
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A paper-based calorimetric microfluidics platform for bio-chemical sensing
Benyamin Davaji1, Chung Hoon Lee1
1Nanoscale Devices Laboratory, Marquette University, Milwaukee, WI 53233, United States.
Biosensors & Bioelectronics
|April 10, 2014
Summary
A new paper-based micro-calorimetric method offers precise biochemical detection by measuring temperature changes. This approach, tested on glucose, DNA, and biotin, shows less than 2% error for glucose detection, extending paper-based analytical capabilities.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Microfluidics
Background:
- Paper-based analytical devices (PADs) currently rely on colorimetric and electrochemical detection.
- Integrating thermal detection offers a novel modality for PADs.
- Biochemical reactions and binding events generate measurable temperature changes.
Purpose of the Study:
- To present a novel paper-based micro-calorimetric method for biochemical detection.
- To demonstrate the feasibility of calorimetric detection as an extension to existing PADs.
- To evaluate the performance of the micro-calorimetric system for specific analytes.
Main Methods:
- Development of a paper-based micro-calorimetric system.
- Measurement of reaction/binding temperatures for glucose/glucose oxidase, DNA/hydrogen peroxide, and biotin/streptavidin.
- Comparison of calorimetric glucose detection with a commercial electrochemical glucose meter using standard calibration samples.
Main Results:
- Calorimetric detection of glucose achieved a measurement error below 2%.
- Demonstrated feasibility for detecting varying DNA concentrations (0.9–7.3 mg/mL) via temperature changes.
- Observed temperature changes in biotin/streptavidin binding events.
Conclusions:
- Paper-based micro-calorimetry is a viable and accurate method for biochemical detection.
- This technique expands the detection capabilities of paper-based microfluidic devices.
- The calorimetric approach offers a promising alternative or complementary detection strategy for PADs.
