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Fulminant liver failure model with hepatic encephalopathy in the mouse
Tomohide Hori1, Feng Chen1, Ann-Marie T Baine1
1Department of Neuroscience, Mayo Clinic in Florida, Jacksonville, FL 32224, USA (Tomohide Hori, Feng Chen, Ann-Marie T. Baine, Lindsay B. Gardner).
Annals of Gastroenterology
|April 10, 2014
Summary
Azoxymethane (AOM) provides a reliable model for fulminant liver failure (FLF) in mice, but requires careful body temperature (BT) control. Other models may also be useful depending on research needs.
Area of Science:
- Hepatology
- Toxicology
- Animal Models
Background:
- Fulminant liver failure (FLF) poses significant clinical challenges.
- Developing reliable animal models is crucial for understanding FLF pathogenesis and testing therapies.
Purpose of the Study:
- To establish and evaluate a murine model for inducing fulminant liver failure (FLF).
- To compare the efficacy of azoxymethane (AOM) and galactosamine (Gal) + tumor necrosis factor-alpha (TNFα) for FLF induction.
- To assess the impact of body temperature (BT) control and hydration on survival and disease parameters.
Main Methods:
- Male C57BL/6 mice were divided into control, AOM-treated, and Gal+TNFα-treated groups.
- The effects of body temperature (BT) control on survival were investigated in all groups.
- Histopathological, biochemical, coagulation (PT-INR), and neurological assessments were performed.
Main Results:
- Azoxymethane (AOM) treatment resulted in significant survival differences with and without BT control.
- Both AOM and Gal+TNFα models showed consistent FLF histopathology with BT care.
- Significant differences in liver enzymes (AST) and PT-INR were observed between models and hydration levels.
Conclusions:
- Azoxymethane (AOM) is a viable option for creating a murine FLF model, necessitating specialized care and BT management.
- The choice of FLF model depends on specific research objectives.
- Meticulous animal care and close monitoring are essential for successful FLF modeling.
