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Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols
Published on: October 18, 2016
Identification and functional characterization of mononuclear cells by flow cytometry
1Department of Immunology/Microbiology, Rush-Presbyterian-St Luke's Medical Center, Chicago, Ill.
Archives of Pathology & Laboratory Medicine
|June 1, 1989
Summary
Advancements in flow cytometry enable multiparameter analysis for clinical applications. Establishing quality control for sample preparation, staining, and data analysis is crucial for accurate characterization of peripheral blood cells.
Area of Science:
- Immunology
- Biotechnology
- Clinical Diagnostics
Background:
- Flow cytometry technology has advanced significantly with new monoclonal antibodies, fluorescent labels, and software for multiparameter analysis.
- Clinical applications necessitate robust standards and quality control (QC) procedures.
Purpose of the Study:
- To outline essential elements for establishing flow cytometry standards and QC procedures.
- To explore multiparameter flow cytometry for characterizing peripheral blood mononuclear cells (PBMCs).
Main Methods:
- Described basic elements for establishing flow cytometry standards and QC.
- Utilized two-color flow cytometry for analyzing T cells, B cells, and natural killer (NK) cells.
Main Results:
- Established procedures for sample preparation, staining, instrument calibration, and data analysis.
- Demonstrated the utility of two-color analysis for PBMC characterization.
Conclusions:
- Multiparameter flow cytometry provides critical insights into cell biology.
- This technique offers significant diagnostic information for T, B, and NK cells.
