Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Vaccine Production01:23

Vaccine Production

144
Vaccine production involves a sequence of upstream and downstream processes to generate a safe and effective immunological product. It begins with cultivating microorganisms, such as viruses or bacteria, to obtain antigenic material. For viral vaccines, mammalian host cells are grown in bioreactors and subsequently infected with the target virus. The virus replicates within the host cells, which are lysed to release viral particles. This lysate is then clarified through filtration or...
144
Vaccinations01:51

Vaccinations

44.1K
Overview
44.1K
Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

1.3K
Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
1.3K
Vaccines01:21

Vaccines

98
Vaccines are among the most effective tools in preventive medicine, designed to prepare the immune system to recognize and combat infectious agents. By introducing antigens—substances that the immune system identifies as foreign—vaccines stimulate an adaptive immune response that leads to immunological memory. This immunological memory enables the body to mount a faster and more effective response upon future exposures to the actual pathogen.Vaccines can be categorized based on the...
98
Outer Layers of the Cell Envelope01:18

Outer Layers of the Cell Envelope

1.5K
The outermost layers of prokaryotic cells play a critical role in their survival, virulence, and interaction with the environment. These layers, often composed of polysaccharides, polypeptides, or proteins, form protective and adhesive structures that vary in organization and function.Capsules and Slime LayersCapsules are highly organized, tightly bound layers that firmly attach to the bacterial cell wall. Capsules are usually made of polysaccharides, though some are made of polypeptides. These...
1.5K
COP Coated Vesicles00:59

COP Coated Vesicles

12.6K
Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of...
12.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Teplizumab (Tzield) for the delay of type 1 diabetes.

JAAPA : official journal of the American Academy of Physician Assistants·2026
Same author

Detection of an arbitrary number of communities in a block spin Ising model.

PloS one·2026
Same author

Flexor Digitorum Longus Strain in a Spinal Cord Injury Patient: A Case Report of a Rare Diagnosis.

Cureus·2026
Same author

"But Some People Still Think That Men Cannot be Raped": A Qualitative Study on Portuguese Judges' Perceptions Regarding Rape Perpetrated by Women Against Adult Men.

International journal of sexual health : official journal of the World Association for Sexual Health·2026
Same author

Improving angiogenesis ameliorates the efficacy of ASO-based exon skipping for the treatment of Duchenne muscular dystrophy.

Molecular therapy. Nucleic acids·2026
Same author

How to do (or not to do)… asset mapping in community health.

Health policy and planning·2026

Related Experiment Video

Updated: May 1, 2026

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria
06:38

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria

Published on: December 8, 2023

1.9K

Bacterial outer membrane vesicles and vaccine applications.

Reinaldo Acevedo1, Sonsire Fernández1, Caridad Zayas1

  • 1Finlay Institute , Havana , Cuba.

Frontiers in Immunology
|April 10, 2014
PubMed
Summary

Outer membrane vesicles (OMVs) are versatile nano-structures with significant potential for vaccine development. Research shows OMVs can be engineered to deliver various antigens, demonstrating broad applicability in creating novel vaccine candidates.

Keywords:
Bordetella pertussisMycobacterium tuberculosisNeisseria meningitidisVibrio choleraeadjuvantouter membrane vesiclesproteoliposomesvaccines

More Related Videos

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain
08:07

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

Published on: July 25, 2022

2.4K
Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity
07:26

Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity

Published on: March 31, 2021

4.7K

Related Experiment Videos

Last Updated: May 1, 2026

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria
06:38

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria

Published on: December 8, 2023

1.9K
A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain
08:07

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

Published on: July 25, 2022

2.4K
Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity
07:26

Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity

Published on: March 31, 2021

4.7K

Area of Science:

  • Vaccinology
  • Nanotechnology
  • Microbiology

Background:

  • Outer membrane vesicles (OMVs) have been utilized in vaccines for over two decades, primarily against Neisseria meningitidis serogroup B.
  • These nano-sized structures possess inherent immunomodulatory properties and serve as effective platforms for antigen delivery.

Purpose of the Study:

  • To review the diverse applications of OMVs in research and development.
  • To present examples of OMV development and evaluation at the Finlay Institute.
  • To highlight the versatility of OMVs beyond meningococcal vaccines.

Main Methods:

  • Development of a Good Manufacturing Practice (GMP) compliant detergent extraction process for OMV production.
  • Production of OMVs from various bacterial species including Neisseria meningitidis, Vibrio cholerae, Bordetella pertussis, and mycobacteria.
  • Evaluation of OMV immunogenicity through antibody induction and functional assays in preclinical models.

Main Results:

  • Successful production of detergent-extracted OMVs (dOMVs) from multiple serogroups of N. meningitidis (B, A, W, X).
  • Demonstrated efficacy of the extraction process for OMVs from non-meningococcal pathogens like V. cholerae and B. pertussis.
  • Preclinical studies confirmed the immunogenicity and protective potential of OMVs, including their use with non-neisserial antigens.

Conclusions:

  • OMV technology is more versatile than initially anticipated.
  • OMVs represent a significant and adaptable platform for the development of diverse vaccine candidates.
  • Continued research and development of OMVs hold promise for addressing various infectious diseases and other immunological applications.