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Updated: May 1, 2026

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
High-efficient nitrogen removal by coupling enriched autotrophic-nitrification and aerobic-denitrification
Shiqiang Zou1, Shuo Yao2, Jinren Ni2
1Department of Environmental Engineering, Peking University, The Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, China; Shenzhen Key Laboratory for Heavy Metal Pollution Control and Reutilization, School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055, China.
Abstract:
This study paid particular attention to total nitrogen removal at low temperature (10°C) by excellent coupling of enriched autotrophic nitrifying and heterotrophic denitrifying consortiums at sole aerobic condition. The maximum specific nitrifying rate of the nitrifying consortium reached 8.85mgN/(gSSh). Further test in four identical lab-scale sequencing batch reactors demonstrated its excellent performance for bioaugmentation in potential applications. On the other hand, the aerobic denitrifying consortium could achieve a specific denitrifying rate of 32.93mgN/(gSSh) under dissolved oxygen of 1.0-1.5mg/L at 10°C. Coupling both kinds of consortiums was proved very successful for a perfect total nitrogen (TN) removal at COD/N of 4 and dissolved oxygen of 1.5-4.5mg/L, which was hardly reached by any single consortium reported previously. The encouraging results from coupling aerobic consortiums implied a huge potential in practical treatment of low-strength domestic wastewater (200-300mg/L COD) during wintertime.
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