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Sensitive sulfide sensor with a trypsin-stabilized gold nanocluster.

Jun Fan1, Ruiping Li, Pingping Xu

  • 1The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou College of Chemistry, Chemical Engineering and Materials Science, Soochow University.

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
|April 11, 2014
PubMed
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We developed a sensitive fluorescent gold nanocluster sensor for detecting sulfide. This novel sensor, stabilized by trypsin, offers a facile method for analyzing sulfide in real samples and persulfate.

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Area of Science:

  • Nanotechnology
  • Analytical Chemistry
  • Biochemistry

Background:

  • Fluorescent gold nanoclusters are promising for sensing applications.
  • Sulfide is an important analyte in various fields, necessitating sensitive detection methods.
  • Developing selective and sensitive sensors for sulfide remains a challenge.

Purpose of the Study:

  • To synthesize a trypsin-stabilized fluorescent gold nanocluster.
  • To develop a fluorescence-based sensor for sulfide detection utilizing fluorescence quenching.
  • To explore the sensor's application in real sample analysis and indirect persulfate detection.

Main Methods:

  • Synthesis of trypsin-stabilized gold nanoclusters.
  • Investigation of the interaction between sulfide and gold nanoclusters leading to fluorescence quenching.
  • Development and calibration of the fluorescence sensor for sulfide.
  • Analysis of real samples and determination of persulfate using the developed sensor.

Main Results:

  • A trypsin-stabilized fluorescent gold nanocluster was successfully synthesized.
  • Sulfide caused significant fluorescence quenching of the nanocluster, forming the basis of the sensor.
  • The sensor exhibited linear response to sulfide from 50 nM to 8 μM with a detection limit of 5.5 nM.
  • Good recoveries were achieved in real sample analysis, and the sensor was adapted for indirect persulfate detection.

Conclusions:

  • A facile and highly sensitive fluorescence sensor for sulfide analysis was developed based on gold nanoclusters.
  • The sensor demonstrated excellent performance in real samples and showed potential for indirect persulfate detection.
  • The study provides a robust platform for sulfide quantification and related analytical challenges.