[Validation of a liquid chromatography method for rifampicin determination in human plasma]
Luis Moreno-Exebio1, Miguel Grande-Ortiz1
1Centro Nacional de Control de Calidad, Instituto Nacional de Salud, Lima, Perú
Objectives:
To validate the high-performance liquid chromatography method (HPLC) for rifampicin (RFP) determination in human plasma.
Materials And Methods:
A HPLC method for RFP determination in plasma was developed. The separation was performed by reversed-phase chromatography with C18 column and a mobile phase composed of a mixture of acetonitrile and monobasic potassium phosphate buffer solution 0.05 M (38:62 v/v) at 335 nm in which standard rifampicin quinone (RFP-QN) was used.
Results:
The retention times of RFP and RFP-QN were 7.81 and 12.26 minutes, respectively. The trial was linear from 0.5 to 250 ug/mL. The evaluated parameters of precision, accuracy, selectivity, linearity, and recovery complied with the established international standards for validation of bioanalytical methods.
Conclusions:
The developed HPLC method is simple, specific, sensitive, selective and linear for a wide range of RFP concentrations in plasma.
