Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 1, 2026

Cell Tracking Using Photoconvertible Proteins During Zebrafish Development
07:19

Cell Tracking Using Photoconvertible Proteins During Zebrafish Development

Published on: September 28, 2012

11.1K

In vivo cell tracking using PhOTO zebrafish.

William P Dempsey1, Hanyu Qin, Periklis Pantazis

  • 1Department of Biosystems Science and Engineering (D-BSSE), ETH Zurich, Mattenstrasse 26, 4058, Basel, Switzerland.

Methods in Molecular Biology (Clifton, N.J.)
|April 11, 2014
PubMed
Summary

Researchers developed new transgenic zebrafish lines for advanced cell tracking during dynamic biological processes. These PhOTO (photoconvertible optical tracking of…) zebrafish enable precise, noninvasive monitoring of cell behavior and interactions in vivo.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Piezo1-mediated mechanohydraulic control of cell volume drives cardiac morphogenesis.

Science advances·2026
Same author

Regulatory Effects of <i>Lactobacillus crispatus</i> and <i>Lactobacillus rhamnosus</i> on the Formation and Composition of <i>Gardnerella</i> Biofilms.

Microorganisms·2026
Same author

Context-specific regulatory genetic variation in MTOR dampens neutrophil-T cell crosstalk in pneumonia-associated sepsis.

Nature communications·2026
Same author

Negative-Valence Neurons in the Larval Zebrafish Pallium.

bioRxiv : the preprint server for biology·2025
Same author

The circulating cell-free DNA landscape in sepsis is dominated by impaired liver clearance.

Cell genomics·2025
Same author

Hyperspectral oblique plane microscopy enables spontaneous, label-free imaging of biological dynamic processes in live animals.

Proceedings of the National Academy of Sciences of the United States of America·2024

Area of Science:

  • Development of novel transgenic animal models for biological research.
  • In vivo imaging and cell tracking technologies.

Background:

  • Accurate cell tracking is crucial for understanding dynamic biological processes like development and disease.
  • Existing in vivo cell tracking methods have limitations in specificity and ease of use.

Purpose of the Study:

  • To generate and validate novel transgenic zebrafish lines for advanced in vivo cell tracking.
  • To enable noninvasive, high-contrast monitoring of cellular dynamics during complex biological events.

Main Methods:

  • Generation of transgenic zebrafish lines expressing fluorescent proteins (Dendra2 fusions).
  • Utilizing irreversible green-to-red photoconversion for targeted cell selection.
  • Employing fluorescence imaging for monitoring nuclear and plasma membrane labeling.

More Related Videos

Two-Photon-Based Photoactivation in Live Zebrafish Embryos
09:10

Two-Photon-Based Photoactivation in Live Zebrafish Embryos

Published on: December 24, 2010

11.2K
Single-cell Photoconversion in Living Intact Zebrafish
09:49

Single-cell Photoconversion in Living Intact Zebrafish

Published on: March 19, 2018

5.4K

Related Experiment Videos

Last Updated: May 1, 2026

Cell Tracking Using Photoconvertible Proteins During Zebrafish Development
07:19

Cell Tracking Using Photoconvertible Proteins During Zebrafish Development

Published on: September 28, 2012

11.1K
Two-Photon-Based Photoactivation in Live Zebrafish Embryos
09:10

Two-Photon-Based Photoactivation in Live Zebrafish Embryos

Published on: December 24, 2010

11.2K
Single-cell Photoconversion in Living Intact Zebrafish
09:49

Single-cell Photoconversion in Living Intact Zebrafish

Published on: March 19, 2018

5.4K
  • Applying the PhOTO zebrafish system for in vivo cell tracking during dynamic events.

    • Main Results:

    • Successful generation of PhOTO zebrafish lines.
    • Demonstration of effective photoconversion and in vivo cell tracking.
    • High-contrast, specific cell selection simplifies tracking and segmentation.
    • PhOTO lines are suitable for studying gastrulation, regeneration, and tumorigenesis.

    Conclusions:

    • PhOTO zebrafish lines offer a powerful, noninvasive tool for in vivo cell tracking.
    • The system facilitates detailed analysis of cell dynamics and interactions.
    • This technology advances the study of developmental biology, tissue regeneration, and cancer progression.