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Updated: May 1, 2026

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
A raspberry bushy dwarf virus isolate from Ecuadorean Rubus glaucus contains an additional RNA that is a
D F Quito-Avila1, M A Ibarra, R Alvarez
1Centro de Investigaciones Biotecnologicas del Ecuador, Escuela Superior Politecnica del Litoral (CIBE-ESPOL), Km 30.5 Via Perimetral Campus Gustavo Galindo. Apartado 09-01-5863, Guayaquil, Ecuador, diego.quito.avila@gmail.com.
Abstract:
Sequencing of the complete genome of a raspberry bushy dwarf virus isolate from Rubus glaucus in Ecuador revealed that its RNA-1 and RNA-2 were 5449 and 2231 nucleotides (nt) long, respectively, and phylogenetically closest to isolates from Sweden and Slovenia. In dsRNA analysis of infected plants, an additional band of 3 kbp was observed. Sequencing of this band revealed that it was 3279 nt long. BLAST searches revealed that this band contained a modified version of RNA-2, which consisted of RNA-2 (2231 nt) plus an additional 1048-nt fragment that was concatenated in a reverse-complement fashion to its 5' terminus.
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