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Repair of bleomycin-induced DNA double-strand breaks in Vicia faba
K J Angelis1, J Velemínský, R Rieger
1Institute of Experimental Botany CSAV, Prague, Czechoslovakia.
Mutation Research
|June 1, 1989
Abstract:
As detected by neutral DNA elution, bleomycin induced at the concentrations tested (5, 10 and 50 micrograms/ml) DNA double-strand breaks (dsbs) in in vitro cultured embryos of V. faba. Most of these breaks were repaired during a 4-h incubation period after treatment. Dsbs also occurred after treatment with 2.5 and 5 mM of N-methyl-N-nitrosourea (MNU) but in contrast to those induced by bleomycin, these dsbs remained unrepaired during the 4-h incubation period following the treatment.