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Updated: May 1, 2026

mRNA Interactome Capture from Plant Protoplasts
Published on: July 28, 2017
Single-stranded nucleic acid binding in Arabidopsis thaliana cold shock protein is cold shock domain dependent
Ashutosh Mani1, Dwijendra K Gupta
1a Center of Bioinformatics, Institute of Interdisciplinary Studies, University of Allahabad , Allahabad 211002 , India.
Abstract:
Cold shock proteins (CSPs) are ancient nucleic acid-binding proteins and well conserved from bacteria to animals as well as plants. In prokaryotes, CSPs possess a single cold shock domain (CSD) while animal CSPs, flanked by N- and C-terminal domains, are commonly named Y-box proteins. Interestingly, the plants CSPs contain auxiliary C-terminal domains in addition to their N-terminal CSD. The CSPs have been shown to play important role in development and stress adaptation in various plant species. The objective of this study was to find out the possible nucleic acid-binding affinities of whole CSP as well as independent domains, so that role of each individual domain may be revealed in Arabidopsis thaliana, the model plant species. The structure of CSP 3 protein from A. thaliana was modeled by homology-based approach and docking was done with different nucleic acid types.
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