Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Formation of the Platelet Plug

Formation of the Platelet Plug

The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

An In Vitro Study of Embolus Migration in an Anatomical Cerebrovascular Model.

Journal of biomechanical engineering·2026

Same author

Red Blood Cell Deformation in a Microfluidic Sudden Expansion at Supraphysiological Strain Rates.

Biophysical journal·2026

Same author

Mechanical compaction alters microstructural and magnetic resonance imaging properties of acute ischemic stroke clots.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism·2026

Same author

Monoclonal Antibodies Targeting Bacterial Infections: A Broad Review of the Field.

BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy·2026

Same author

Computational fluid dynamics assessment of altered hemodynamics in the Circle of Willis during acute ischemic stroke and the impact of cerebral collateral development.

Biomechanics and modeling in mechanobiology·2026

Same author

Anti-Biofilm Properties of Polyurethane Biomaterials Tethered With Small Molecules via Polyethylene Glycol Linker.

Journal of biomedical materials research. Part A·2026

Search research articles

Related Experiment Video

Updated: May 1, 2026

Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells

Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells

Published on: October 27, 2009

Platelet adhesion to polyurethane urea under pulsatile flow conditions.

Michael A Navitsky¹, Joshua O Taylor, Alexander B Smith

¹Department of Bioengineering, Pennsylvania State University, University Park, PA, USA.

Artificial Organs

|April 12, 2014

Summary

Platelet adhesion to polyurethane urea surfaces in blood-contacting devices decreases with increasing shear rate under pulsatile flow. Understanding this relationship helps predict thrombus formation in cardiovascular devices.

Keywords:

Fluid dynamics Platelet adhesion Polyurethane Pulsatile flow Rotating disk Shear stress

More Related Videos

In Vitro and In Vivo Model to Study Bacterial Adhesion to the Vessel Wall Under Flow Conditions

In Vitro and In Vivo Model to Study Bacterial Adhesion to the Vessel Wall Under Flow Conditions

Published on: June 11, 2015

Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets

Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets

Published on: April 9, 2018

Related Experiment Videos

Last Updated: May 1, 2026

Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells

Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells

Published on: October 27, 2009

In Vitro and In Vivo Model to Study Bacterial Adhesion to the Vessel Wall Under Flow Conditions

In Vitro and In Vivo Model to Study Bacterial Adhesion to the Vessel Wall Under Flow Conditions

Published on: June 11, 2015

Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets

Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets

Published on: April 9, 2018

Area of Science:

Biomaterials Science
Cardiovascular Engineering
Hemodynamics

Background:

Platelet adhesion to polyurethane urea surfaces is a critical initial step in thrombus formation within blood-contacting cardiovascular devices.
Strong platelet adhesion is observed on polyurethane surfaces at shear rates below approximately 500 s(-1).

Purpose of the Study:

To investigate the characteristics of platelet adhesion to polyurethane urea surfaces under time-varying shear stress.
To determine the impact of steady and pulsatile flow conditions on platelet adhesion dynamics.

Main Methods:

Utilized a rotating disk system to simulate blood flow conditions.
Exposed polyurethane urea surfaces to platelet-rich bovine plasma under controlled steady and pulsatile shear rates for 2 hours.
Quantified platelet adhesion using confocal microscopy of immunofluorescently labeled platelets.

Main Results:

Platelet adhesion exhibited an exponential decay with increasing shear rate under pulsatile flow conditions.
Adhesion levels were dependent on peak platelet flux and shear rate, irrespective of the specific rotational waveform.
Results indicate a significant influence of shear rate dynamics on platelet-material interactions.

Conclusions:

Platelet adhesion to polyurethane urea is shear-rate dependent and influenced by flow pulsatility.
The findings provide valuable data for predicting areas prone to thrombus formation in devices like ventricular assist devices.
This research contributes to the development of safer and more effective blood-contacting cardiovascular implants.