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Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
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CMTM5 exhibits tumor suppressor activity through promoter methylation in oral squamous cell carcinoma

Heyu Zhang1, Xu Nan2, Xuefen Li1

  • 1Central Laboratory, Peking University School of Stomatology, Beijing, China.

Biochemical and Biophysical Research Communications
|April 12, 2014
PubMed

Insights

CKLF-like MARVEL transmembrane domain-containing member 5 (CMTM5) acts as a tumor suppressor in oral squamous cell carcinoma (OSCC). Its down-regulation, due to promoter methylation, inhibits OSCC progression and tumor formation.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Oral squamous cell carcinoma (OSCC) is a prevalent head and neck malignancy.
  • CKLF-like MARVEL transmembrane domain-containing member 5 (CMTM5) is recognized as a potential tumor suppressor in various cancers.

Purpose of the Study:

  • To investigate the expression and functional role of CMTM5 in oral squamous cell carcinoma.
  • To determine the mechanism of CMTM5 down-regulation in OSCC.

Main Methods:

  • Analysis of CMTM5 expression in OSCC cell lines and patient tumor samples.
  • Investigation of promoter methylation as a cause for CMTM5 down-regulation.
  • Assessment of CMTM5 restoration using 5-aza-2'-deoxycytidine.
  • Evaluation of CMTM5-v1 ectopic expression effects on OSCC cell proliferation, migration, and apoptosis in vitro.
  • In vivo tumor formation assays.

Main Results:

  • CMTM5 expression was significantly down-regulated in OSCC cell lines and tumor tissues.
  • Promoter methylation was identified as the mechanism for CMTM5 down-regulation.
  • Demethylating agent treatment restored CMTM5 expression.
  • Ectopic CMTM5-v1 expression suppressed OSCC cell proliferation and migration, and induced apoptosis.
  • CMTM5-v1 inhibited tumor formation in vivo.

Conclusions:

  • CMTM5 functions as a tumor suppressor in oral squamous cell carcinoma.
  • Promoter methylation-induced down-regulation of CMTM5 contributes to OSCC development.
  • CMTM5 represents a potential therapeutic target for oral squamous cell carcinoma.
Keywords:
CMTM5Oral squamous cell carcinomaPromoter methylationTumor suppressor

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