Nitroblue tetrazolium reduction in monocytes and monocyte-derived macrophages. Effect of oxidative burst stimulants and interferons

The ability to mount an oxidative burst (OB) in response to medium, zymosan and phorbol myristate acetate (PMA) was assessed in human blood monocytes cultured for 1 day (MO) and monocyte-derived macrophages cultured for 10 days (MDM). Further, the effect of recombinant interferons (IFNs) on OB generation was examined. The OB was measured as a reduction of nitroblue tetrazolium (NBT). Unstimulated and stimulated NBT reduction per cell nucleus and the ratio of stimulated/unstimulated NBT reduction was not significantly different in cells cultured for 1 and 10 days. In MO, IFN-gamma stimulated the OB when co-stimulated with zymosan or PMA. IFN-alpha reduced MO adherence. When the lower adherence was corrected for, IFN-alpha enhanced NBT reduction. In MDM, a high concentration of IFN-gamma stimulated the OB without co-stimulation, in lower concentrations the presence of a co-stimulant was necessary for OB stimulation. IFN-alpha/beta enhanced the OB in response to PMA, suggesting that IFN-alpha/beta has a role in macrophage activation.