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Thyroid hormone response elements in pituitary genes.

J A Ahlquist1, J A Franklyn, M C Sheppard

  • 1Department of Medicine, University of Birmingham, Queen Elizabeth Hospital, U.K.

Biochimica Et Biophysica Acta
|July 7, 1989
PubMed
Summary
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Thyroid hormones impact gene transcription in the pituitary. Researchers found that the thyroid response elements in thyrotrophin and prolactin genes differ from that in the rat growth hormone gene.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Gene Regulation

Background:

  • Thyroid hormones are crucial regulators of gene transcription in the anterior pituitary gland.
  • A known thyroid hormone response element (TRE) in the rat growth hormone gene mediates thyroid hormone effects on transcription.
  • The structural characteristics of TREs in other pituitary genes are not fully understood.

Purpose of the Study:

  • To investigate the presence of sequences similar to the rat growth hormone TRE in the regulatory regions of the thyrotrophin alpha and beta subunit and prolactin genes.
  • To determine if the identified TREs in these genes share sequence homology with the known rat growth hormone TRE.

Main Methods:

  • Analysis of the 5' flanking regions of the thyrotrophin alpha and beta subunit and prolactin genes.

Related Experiment Videos

  • Utilizing Southern blotting to detect sequence similarities to the rat growth hormone TRE.

    • Main Results:

    • Southern blotting revealed no sequences similar to the rat growth hormone TRE in the examined 5' flanking regions of the thyrotrophin subunit and prolactin genes.
    • This indicates a potential difference in the DNA-binding sites for thyroid hormones between the growth hormone gene and the thyrotrophin and prolactin genes.

    Conclusions:

    • The thyroid response elements in the thyrotrophin and prolactin genes likely differ in sequence from the TRE found in the rat growth hormone gene.
    • This suggests distinct molecular mechanisms for thyroid hormone regulation of different pituitary genes.