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Cloning of Plasmodium yoelii genes expressing three different sporozoite-specific antigens
A Wortman1, P Rogers, Y Charoenvit
1Infectious Diseases Program Center, Naval Medical Research Institute, Bethesda, Maryland 20814-5055.
Microbial Pathogenesis
|March 1, 1989
Abstract:
Genomic DNA isolated from Plasmodium yoelii (strain #17XNL) was prepared by partial digestion and cloned in Escherichia coli TB-1 with pUC18 plasmid. Antigen-producing recombinants were detected by a battery of monoclonal antibodies against antigens of the sporozoite stages. Four clones producing stage-specific sporozoite antigens were identified. One produced P. yoelii circumsporozoite protein, and three produced other P. yoelii sporozoite antigens.