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Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
Published on: December 11, 2021
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Recent applications of fluorescence correlation spectroscopy in live systems
Radek Macháň1, Thorsten Wohland1
1Departments of Biological Sciences and Chemistry and Centre for BioImaging Sciences, National University of Singapore, Singapore 117557, Singapore.
FEBS Letters
|April 15, 2014
Summary
Fluorescence correlation spectroscopy (FCS) offers sensitive, quantitative analysis of molecules in live biological samples. This review highlights recent FCS applications for studying protein concentrations, diffusion, and interactions in vivo.
Area of Science:
- Biophysics
- Life Sciences
- Molecular Biology
Background:
- Fluorescence correlation spectroscopy (FCS) is a powerful biophysical technique.
- It offers single-molecule sensitivity for analyzing biological samples at physiological concentrations.
- FCS provides quantitative data on molecular concentrations, diffusion, transport, and interactions.
Purpose of the Study:
- To review recent applications of FCS in live biological samples.
- To emphasize advancements in FCS over the last five years.
- To provide an overview of FCS capabilities for addressing biologically relevant questions.
Main Methods:
- Focus on fluorescence intensity fluctuations.
- Analysis of single molecules within biological systems.
- Application in live organisms and physiological conditions.
Main Results:
- FCS enables measurement of proteins without overexpression.
- Quantitative data on diffusion coefficients and molecular interactions are obtained.
- Recent studies demonstrate broad applicability in live samples.
Conclusions:
- FCS is a versatile and widely applicable technique in biophysics.
- Recent advancements have expanded its use in live biological systems.
- FCS continues to be crucial for answering complex biological questions.
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