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Updated: May 1, 2026

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy
Published on: December 16, 2021
Arginine methylation of the cellular nucleic acid binding protein does not affect its subcellular localization but
Hung-Ming Wei1, Huan-Hsuan Hu1, Gia-Yun Chang1
1Department of Biomedical Sciences, Chung Shan Medical University, Taichung, Taiwan, ROC.
Abstract:
Cellular nucleic acid binding protein (CNBP) contains seven zinc finger (ZF) repeats and an arginine and glycine (RG) rich sequence between the first and the second ZF. CNBP interacts with protein arginine methyltransferase PRMT1. Full-length but not RG-deleted or mutated CNBP can be methylated. Treatment with a methylation inhibitor AdOx reduced CNBP methylation, but did not affect the concentrated nuclear localization of CNBP. Nevertheless, arginine methylation of CNBP appeared to interfere with its RNA binding activity. Our findings show that arginine methylation of CNBP in the RG motif did not change the subcellular localization, but regulated its RNA binding activity.
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