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Branchial cleft cysts. Histologic and immunohistochemical aspects
T Ninomiya1, M Hosaka, H Higashiyama
1Department of Oral Surgery, Asahi University, School of Dentistry, Gifu, Japan.
Acta Histochemica
|January 1, 1989
Summary
Immunohistochemistry revealed specific protein expressions in branchial cleft cysts and branchiogenic carcinoma. Findings highlight keratin, CEA, and Factor VIII RGA presence, aiding in understanding these developmental abnormalities.
Area of Science:
- Pathology
- Head and Neck Surgery
- Developmental Biology
Background:
- Branchial cleft cysts and branchiogenic carcinoma are congenital anomalies.
- Understanding their cellular composition is crucial for diagnosis and treatment.
Purpose of the Study:
- To investigate the immunohistochemical profiles of branchial cleft cysts and branchiogenic carcinoma.
- To characterize keratin, immunoglobulin, CEA, Factor VIII RGA, and lysozyme expression.
- To determine lectin binding patterns in these lesions.
Main Methods:
- Immunohistochemical analysis of 8 branchial cleft cysts and 1 branchiogenic carcinoma.
- Detection of keratin, immunoglobulins (IgG, IgA, IgM), CEA, Factor VIII RGA, and lysozyme.
- Assessment of lectin binding patterns.
Main Results:
- Epithelial cells showed slight keratin expression and lectin binding similar to oral squamous epithelium.
- Columnar epithelial cells expressed CEA; most cystic epithelia lacked lysozyme.
- Endothelial cells were UEA-1 lectin positive and Factor VIII RGA positive.
- Lymphoid structures contained lysozyme-positive cells and lymphocytes expressing IgG, IgA, or IgM.
Conclusions:
- Immunohistochemistry provides insights into the cellular markers of branchial cleft cysts and branchiogenic carcinoma.
- The findings contribute to differentiating these entities and understanding their origins.