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Updated: May 1, 2026

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
Published on: September 20, 2016
Microbial lipid produced by Yarrowia lipolytica QU21 using industrial waste: a potential feedstock for biodiesel
Jandora Severo Poli1, Mirra Angelina Neres da Silva2, Ezequias P Siqueira3
1Department of Microbiology, Immunology and Parasitology, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite 500 sala 154, CEP 90050-170 Porto Alegre, RS, Brazil; Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, CEP 31270-901 Belo Horizonte, MG, Brazil.
Abstract:
This study aimed to evaluate the effect of medium composition and culture conditions on lipid content, fatty acid profile and biomass production by the yeast Yarrowia lipolytica QU21. Lipid production by the yeast growing on glycerol/(NH4)2SO4 (10%/0.1%) reached 1.48g/L (30.1% according to total cell dry weight). When glycerol was replaced by crude glycerol (industrial waste), the lipid yield was 1.27g/L, with no significant difference. Some particular fatty acids were found when crude glycerol was combined with fresh yeast extract (FYE, brewery waste), as linolenic acid (C18:3n3), eicosadienoic acid (C20:2), eicosatrienoic acid (C20:3n3) and eicosapentaenoic acid (C20:5n3). In addition, the FYE promoted an increase of more than 300% on polyunsaturated fatty acid content (PUFA), which is an undesirable feature for biodiesel production. The fatty acid composition of the oil produced by Y. lipolytica QU21 growing on crude glycerol/(NH4)2SO4 presented a potential use as biodiesel feedstock, with low PUFA content.
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