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[RNA synthesis using T7 phage RNA polymerase: transcription of synthetic DNA templates in solution and on polymer
Bioorganicheskaia Khimiia
|February 1, 1989
Abstract:
Transcription of synthetic DNA by T7 RNA polymerase was used to obtain oligoribonucleotides of defined sequence. The enzyme's ability to transcribe DNA immobilized on hydrazide-sepharose was revealed. DNA templates used in such synthesis can be constructed by means of enzymatic (DNA ligase) or chemical ligation (cyanogen bromide).