Related Experiment Video
Updated: May 1, 2026
Compact Quantum Dots for Single-molecule Imaging
Published on: October 9, 2012
Bright quantum-dot-sized single-chain conjugated polyelectrolyte nanoparticles: synthesis, characterization and
Jie Liu1, Guangxue Feng, Rongrong Liu
1Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore, 117576.
Abstract:
We report a simple method to fabricate quantum-dot-sized nanoparticles (NPs) from poly[9,9-bis((6-N,N,N-trimethylammonium)hexyl)fluorene-alt-co-2,1,3-benzo-xadiazole dibromide] (PFBD). The transmission electron microscope results reveal that the obtained NPs have a mean diameter of ≈4 nm, which is composed of a single PFBD chain. The NPs show bright fluorescence with an emission maximum at ≈636 nm and a quantum yield of ≈26% in water. The fluorescence properties of the NPs are characterized by steady fluorescence microscopy, fluorescence dynamic study and single nanoparticle microscopy, which show superior brightness over commercial quantum dots QD655. The NPs are further conjugated with streptavidin to yield PFBD-SA NPs, which serve as a specific extracellular labeling and imaging probe with high specificity and good photostability.
