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A photoreversible phytochrome affinity column chromatography for putative phytochrome receptor studies
B R Singh1, Y G Chai, D T Robertson
1Department of Chemistry and Biochemistry, Texas Tech University, Lubbock 79409.
Journal of Biochemical and Biophysical Methods
|March 1, 1989
Abstract:
Using a photoreversible phytochrome affinity column, we have isolated a small RNA molecule that binds to the affinity column. The RNA was identified by a A260/A280 ratio of 2.0, hypochromicity, orcinol, ribonuclease A and DNase tests. Agarose gel electrophoresis and circular dichroic spectral characteristics also indicate it to be a small RNA molecule. The successful preparation of a photoreversible phytochrome affinity column, the possible usage of the column and the significance of the RNA binding have been discussed.