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Hepatocyte-stimulating factor III shares structural and functional identity with leukemia-inhibitory factor
1Department of Molecular and Cellular Biology, Roswell Park Memorial Institute, Buffalo, NY 14263.
Journal of Immunology (Baltimore, Md. : 1950)
|August 15, 1989
Summary
Hepatocyte-stimulating factor III (HSF-III), derived from epidermal cells, stimulates acute phase protein production. This cytokine is identical to leukemia-inhibitory factor (LIF), linking liver regulation to hemopoietic cell control.
Area of Science:
- Biochemistry
- Cell Biology
- Immunology
Background:
- Acute phase plasma proteins are crucial for the inflammatory response.
- Cytokines mediate the hepatic production of these proteins.
- Interleukin-6 (IL-6) is a known regulator of acute phase protein synthesis.
Purpose of the Study:
- To investigate the role of hepatocyte-stimulating factor III (HSF-III) in acute phase protein regulation.
- To determine the relationship between HSF-III and other known cytokines, specifically IL-6 and leukemia-inhibitory factor (LIF).
Main Methods:
- Characterization of HSF-III produced by COLO-16 cells.
- Comparison of HSF-III's physicochemical properties and biological activity with LIF.
- Neutralization assays using HSF-III antibodies.
- Analysis of LIF mRNA in COLO-16 cells.
Main Results:
- HSF-III stimulates the synthesis of acute phase plasma proteins, similar to IL-6.
- HSF-III shares physicochemical properties with T cell-derived LIF.
- HSF-III exhibits identical liver-regulating activity to human recombinant LIF (rLIF) on hepatoma cells.
- HSF-III antibodies neutralize LIF activity.
- COLO-16 cells express LIF mRNA characteristic of lectin-stimulated T cells.
Conclusions:
- HSF-III is an epidermal cell-derived form of LIF.
- This finding highlights a close relationship between hepatic acute phase regulation and the control of hemopoietic cell proliferation and differentiation by common cytokines.