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The effect of gas double-dynamic on mass distribution in solid-state fermentation
Hong-Zhang Chen1, Zhi-Min Zhao2, Hong-Qiang Li1
1State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
Enzyme and Microbial Technology
|April 16, 2014
Summary
Investigating mass distribution in solid-state fermentation (SSF) using near-infrared spectroscopy (NIRS) revealed that gas double-dynamic solid-state fermentation (GDSSF) enhances heat transfer and microbial metabolism for improved product homogeneity.
Area of Science:
- Biotechnology
- Biochemical Engineering
Background:
- Mass distribution regularity in solid-state fermentation (SSF) is poorly understood due to substrate heterogeneity and analytical limitations.
- This hinders comprehensive analysis and optimization of SSF processes.
Purpose of the Study:
- To determine the distribution of water, biomass, and fermentation products within the SSF substrate.
- To investigate the impact of gas double-dynamic solid-state fermentation (GDSSF) on heat transfer, microbial activity, and product distribution.
Main Methods:
- Utilized near-infrared spectroscopy (NIRS) with developed models to analyze mass distribution at different substrate depths.
- Compared GDSSF with static aeration solid-state fermentation (SASSF) under controlled environmental conditions.
Main Results:
- GDSSF resulted in lower maximum substrate temperatures (33.9°C vs. 39.5°C) and higher carbon dioxide evolution rates (5.38mg/(hg) vs. 2.48mg/(hg)) compared to SASSF.
- GDSSF achieved more homogeneous product distribution (cellulase activity ratios) across substrate depths by the fifth day compared to SASSF on the seventh day.
Conclusions:
- GDSSF effectively enhances SSF performance by improving heat transfer and microbial metabolism.
- NIRS analysis combined with GDSSF offers a powerful approach to optimize SSF processes and product yields.
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