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Updated: May 1, 2026

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
Functionally defective high-density lipoproteins are related to heightened T-cell activation in vertically
Talía Sainz1, Adriana Ortega-Hernández, Sergio Serrano-Villar
1*Laboratorio de InmunoBiología Molecular, Department of Pediatrics, Instituto de Investigación Sanitaria Hospital General Universitario Gregorio Marañón (IiSGM), Servicio de Pediatría e Instituto de Investigación Sanitaria Hospital Gregorio Marañón, Madrid, Spain; †Laboratorio de Vascular, Hospital Clínico San Carlos, Instituto de Investigación Sanitaria San Carlos (IdISSC), Madrid, Spain; ‡Department of Infectious Diseases, Servicio de Enfermedades Infecciosas e IRYCIS, Hospital Universitario Ramón y Cajal, and IRYCIS, Madrid, Spain; §Department of Pediatrics, Servicio de Pediatría, Hospital General Universitario Gregorio Marañón, Madrid, Spain; ‖Department of Pediatrics, Unidad de Inmunodeficiencias pediátricas, Servicio de Pediatría, Hospital Universitario Doce de Octubre, Madrid, Spain; ¶Department of Pediatrics, Servicio de Pediatría, Hospital de Getafe, Madrid, Spain; #Department of Pediatrics, Servicio de Pediatría, Hospital Carlos III, Madrid, Spain; **Department of Internal Medicine, Servicio de Medicina Interna, Hospital Clínico San Carlos, Madrid, Spain; and ††Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain.
Abstract:
We assessed high-density lipoprotein (HDL) anti-inflammatory properties in a cohort of vertically HIV-infected adolescents. We hypothesized that proatherogenic mechanisms related to inflammation and immune activation during HIV infection may impair HDL functionality and impact on the atherosclerotic burden. Compared with healthy controls, HDL from HIV-infected adolescents presented impaired functionality, as determined by its ability to inhibit monocyte chemotaxis in vitro, which correlated with detectable viral loads (P = 0.044), lower CD4 nadir (P = 0.043), increased levels of CD4 T-cell activation (P = 0.018), higher C-reactive protein (P = 0.009), and a tendency toward thicker carotid intima-media thickness (P = 0.071).
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