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Updated: May 1, 2026
Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
Published on: September 5, 2013
Multiplex PCR for identification of two capsular types in epidemic KPC-producing Klebsiella pneumoniae sequence type
Liang Chen1, Kalyan D Chavda1, Jacqueline Findlay2
1Public Health Research Institute Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA.
Abstract:
We developed a multiplex PCR assay capable of identifying two capsular polysaccharide synthesis sequence types (sequence type 258 [ST258] cps-1 and cps-2) in epidemic Klebsiella pneumoniae ST258 strains. The assay performed with excellent sensitivity (100%) and specificity (100%) for identifying cps types in 60 ST258 K. pneumoniae sequenced isolates. The screening of 419 ST258 clonal isolates revealed a significant association between cps type and K. pneumoniae carbapenemase (KPC) variant: cps-1 is largely associated with KPC-2, while cps-2 is primarily associated with KPC-3.
