Rapid regulation of nuclear proteins by rapamycin-induced translocation in fission yeast
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Published on: September 6, 2024
Lin Ding1, Dana Laor, Ronit Weisman
1Molecular and Computational Biology, University of Southern California, Los Angeles, CA, USA.
Abstract:
Genetic analysis of protein function requires a rapid means of inactivating the gene under study. Typically, this exploits temperature-sensitive mutations or promoter shut-off techniques. We report the adaptation to Schizosaccharomyces pombe of the anchor-away technique, originally designed in budding yeast by Laemmli lab. This method relies on a rapamycin-mediated interaction between the FRB- and FKBP12-binding domains to relocalize nuclear proteins of interest to the cytoplasm. We demonstrate a rapid nuclear depletion of abundant proteins as proof of principle.
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