Related Experiment Videos
Isolation and sequence of lambda gt11 cDNA clones encoding the 5B4 antigen expressed on sprouting neurons
1Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas 75235-9050.
Brain Research. Molecular Brain Research
|June 1, 1989
Summary
Monoclonal antibody 5B4 identifies a neural cell adhesion molecule (NCAM) on developing neurons. This study confirms the 5B4 antigen is rat brain NCAM, crucial for neuronal development.
Area of Science:
- Neuroscience
- Molecular Biology
- Immunology
Background:
- Monoclonal antibody (mAb) 5B4 targets a glycoprotein on sprouting neurons.
- This glycoprotein's molecular weight is approximately 185,000-255,000.
- Its developmental regulation is key to understanding neuronal growth.
Purpose of the Study:
- To identify the 5B4 antigen.
- To determine its relationship with known neural cell adhesion molecules.
- To characterize the epitope recognized by mAb 5B4.
Main Methods:
- Deduction of amino acid sequence from lambda gt11 cDNA clones.
- Sequence analysis and comparison with chick NCAM-ld.
- Epitope mapping of the 5B4 antigen.
Main Results:
- The deduced amino acid sequence of the 5B4 antigen is co-linear with chick NCAM-ld.
- A 75% sequence identity confirms the 5B4 antigen as rat brain NCAM.
- The 5B4 epitope is located at the carboxy-terminus shared by NCAM-ld and NCAM-sd.
Conclusions:
- Monoclonal antibody 5B4 recognizes rat brain Neural Cell Adhesion Molecule (NCAM).
- The 5B4 antigen is a developmentally regulated glycoprotein expressed on sprouting neurons.
- The epitope is conserved in different NCAM isoforms (NCAM-ld and NCAM-sd).