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Updated: May 1, 2026

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
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2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

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Sequencing-by-ligation using oligonucleotide probes with 3'-thio-deoxyinosine

Dan Pu, Jing Chen, Yunfei Bai

    Journal of Biomedical Nanotechnology
    |April 17, 2014
    PubMed
    Summary

    Researchers created a new sequencing-by-ligation (SBL) method using modified oligonucleotide probes on microarrays. This accurate DNA sequencing technique achieves a 40 bp read length and is suitable for automation.

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