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Meiosis II

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Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...

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Meiosis II is the second and final stage of meiosis. It relies on the haploid cells produced during meiosis I, each of which contain only 23 chromosomes—one from each homologous initial pair. Importantly, each chromosome in these cells is composed of two joined copies, and when these cells enter meiosis II, the goal is to separate such sister chromatids using the same microtubule-based network employed in other division processes. The result of meiosis II is two haploid cells, each...

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Related Experiment Video

Updated: May 1, 2026

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Published on: October 11, 2015

Dynamin 2 regulates actin-mediated spindle migration in mouse oocytes.

Qiao-Chu Wang¹, Jun Liu, Zhen-Bo Wang

¹College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.

Biology of the Cell

|April 17, 2014

Summary

Dynamin 2 is crucial for mouse oocyte meiosis, regulating spindle migration and polar body extrusion. Disrupting Dynamin 2 function impairs actin dynamics, leading to failed cell division.

Keywords:

Actin Cytokinesis Dynamin Oocyte Spindle migration

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Area of Science:

Cell Biology
Reproductive Biology
Molecular Biology

Background:

Meiosis in oocytes involves spindle formation and migration for polar body extrusion.
Actin dynamics and related molecules are critical for oocyte cytokinesis.
Dynamin 2, a GTPase, influences actin recruitment and vesicle mobility.

Purpose of the Study:

To investigate the role of Dynamin 2 in mouse oocyte meiosis.
To understand how Dynamin 2 affects spindle behavior and cytokinesis.

Main Methods:

RNA interference (RNAi) to disrupt Dynamin 2 activity.
Inhibitor treatment to block Dynamin 2 function.
Time-lapse and live cell imaging.
Immunofluorescence to assess protein localization and actin dynamics.

Main Results:

Dynamin 2 localizes to the oocyte cortex and spindle.
Disruption of Dynamin 2 caused failure in polar body extrusion and oocyte cytokinesis.
Chromosome separation errors and rejoining were observed.
Decreased actin expression, disrupted actin cap formation, and failed spindle migration occurred.
Profilin and ARP2/3 complex distribution was altered upon Dynamin 2 disruption.

Conclusions:

Dynamin 2 plays a regulatory role in mouse oocyte meiosis.
The protein is essential for spindle migration and polar body extrusion.
Dynamin 2 acts via an actin-dependent pathway to control these meiotic events.