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Related Concept Videos

Septins01:19

Septins

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Septins are protein filaments forming the cytoskeleton along with the microtubules, microfilaments, intermediate filaments, and other accessory proteins. In 1971 while studying the cell division cycle in mutant Saccharomyces cerevisiae Harwell et al. first identified the septin-related genes playing a crucial role in yeast cytokinesis. Fluorescence microscopy revealed that these proteins localize at the budding neck as rings. These ring-like proteins were then named Septins by John Pringle, and...
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Role of Septins01:02

Role of Septins

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Septins are the recently discovered fourth major protein component of the cytoskeleton, along with microfilaments, microtubules, and intermediate filaments. These proteins can associate with other cytoskeletal filaments and carry out varied roles or can be free-floating in the cytoplasm.
Cellular Functions of Septins
Recent studies have revealed the multifaceted roles of septins in various cellular processes such as cytokinesis, ciliogenesis, and neurogenesis. Septins act as scaffolds and...
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Introduction to the Cytoskeleton01:33

Introduction to the Cytoskeleton

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Overview of the Cytoskeleton
The cytoskeleton is a network of protein filaments present within the cell, having three distinct filaments ̶   microfilaments, microtubules, and intermediate filaments. Each has characteristic features that distinguish them, including the dynamics of their assembly and disassembly, mechanical properties, polarity, and the type of molecular motors associated with them. Earlier, they were thought to be present only in eukaryotic cells; however, their...
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Cytoskeletal Coordination in Cell Migration01:32

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A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
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Assembly of Cytoskeletal Filaments01:18

Assembly of Cytoskeletal Filaments

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Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
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Adaptability of Cytoskeletal Filaments01:12

Adaptability of Cytoskeletal Filaments

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The cytoskeleton is a complex dynamic structure performing varied functions based on cellular requirements. The adaptability of the individual filaments in the cytoskeleton determines their ability to perform various functions within the cell. It can undergo rapid reorganization during processes like cell division or remain stable for several hours as in the interphase. The adaptability of these filaments depends on stringent regulatory mechanisms. The microfilament and microtubules of the...
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Related Experiment Video

Updated: May 1, 2026

Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution
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Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution

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Cytoskeleton: septins do the horizontal tango.

Theodore Pham1, Jessica E DiCiccio1, William S Trimble1

  • 1Program in Cell Biology, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada, and Department of Biochemistry, University of Toronto, Toronto, Ontario M5S 1A8, Canada.

Current Biology : CB
|April 17, 2014
PubMed
Summary
This summary is machine-generated.

Researchers visualized septin filament formation in real time for the first time. This provides key insights into the organization of these essential filamentous proteins crucial for cell division.

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Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins
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Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions
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Area of Science:

  • Cell Biology
  • Molecular Biology

Background:

  • Septins are a conserved family of filamentous GTPases.
  • They are essential for various cellular processes, including cytokinesis and membrane organization.
  • Septins assemble into dynamic filaments and higher-order structures at specific cellular locations.

Purpose of the Study:

  • To visualize the dynamic process of septin filament formation in real time.
  • To gain novel insights into the assembly mechanisms and organization of septin filaments.

Main Methods:

  • Utilized advanced live-cell imaging techniques.
  • Employed fluorescently tagged septin proteins to monitor assembly dynamics.

Main Results:

  • Provided the first real-time visualization of septin filament assembly.
  • Revealed dynamic aspects of septin organization and polymerization.
  • Identified key steps in filament formation and stabilization.

Conclusions:

  • The study offers unprecedented insights into septin filament dynamics.
  • Understanding septin organization is critical for comprehending cell division and other fundamental biological processes.
  • These findings lay the groundwork for future research into septin function and regulation.