Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conserved Binding Sites01:49

Conserved Binding Sites

4.1K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
4.1K
Conserved Binding Sites01:49

Conserved Binding Sites

1.1K
1.1K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

6.0K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
6.0K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

16.7K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
16.7K
Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

3.3K
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
3.3K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.1K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proteomic profiling of whole tissue sections in cardiac ATTR amyloidosis reveals increased extracellular matrix remodeling.

Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology·2026
Same author

Integrated omics reveal a unique antibacterial mechanism of action for the small molecule HSI#6.

Current research in microbial sciences·2026
Same author

Confirmation of monotypic immunofluorescence staining by mass spectrometry in a case of proliferative glomerulonephritis.

Histopathology·2026
Same author

FoldDelay web server: an online tool to quantify translation-driven delays in protein native contact formation.

Nucleic acids research·2026
Same author

Belgian recommendations for tissue diagnosis of amyloidosis.

Acta clinica Belgica·2026
Same author

Atomic structures of medin and Aβ fibrils reveal polymorphic remodeling in mixed amyloid systems.

Nature communications·2026
Same journal

UPF3A and UPF3B shape the transcriptome cooperatively yet oppose cell function.

Journal of molecular biology·2026
Same journal

Antibody-secreting cells integrate efficient NMD with non‑canonical UPR signaling to maintain proteostasis and support massive immunoglobulin synthesis.

Journal of molecular biology·2026
Same journal

Small molecule stabilization of diverse amyloidogenic immunoglobulin light chains revealed by hydrogen-deuterium exchange mass spectrometry.

Journal of molecular biology·2026
Same journal

UPF1 at Work: Structural and Mechanistic Insights Into a Master Regulator of Nonsense-Mediated mRNA Decay.

Journal of molecular biology·2026
Same journal

Structural basis for the pro-amyloidogenic action and ligand binding of a novel W72R variant of human apolipoprotein A-I.

Journal of molecular biology·2026
Same journal

Cryo-EM Structure of the C. Elegans Septin Tetramer Reveals a Revised Architecture and Conserved Positional Orthology.

Journal of molecular biology·2026
See all related articles

Related Experiment Video

Updated: May 1, 2026

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
22:27

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Published on: May 6, 2010

414.5K

A genome-wide sequence-structure analysis suggests aggregation gatekeepers constitute an evolutionary constrained

Greet De Baets1, Joost Van Durme1, Frederic Rousseau2

  • 1Switch Laboratory, Flanders Institute for Biotechnology (Vlaams Instituut voor Biotechnologie), 3000 Leuven, Belgium; Switch Laboratory, Department of Cellular and Molecular Medicine, University of Leuven, Herestraat 49, 3000 Leuven, Belgium; Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels, Belgium.

Journal of Molecular Biology
|April 17, 2014
PubMed
Summary
This summary is machine-generated.

Aggregation gatekeepers, charged residues and prolines, are selectively conserved to control protein aggregation. Their presence is evolutionarily driven, not solely by structural constraints, despite a thermodynamic cost to protein stability.

Keywords:
evolutiongatekeepersprotein aggregationthermodynamic stability

More Related Videos

Folding and Characterization of a Bio-responsive Robot from DNA Origami
07:59

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

14.0K
Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
14:06

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

46.1K

Related Experiment Videos

Last Updated: May 1, 2026

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
22:27

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Published on: May 6, 2010

414.5K
Folding and Characterization of a Bio-responsive Robot from DNA Origami
07:59

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

14.0K
Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
14:06

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

46.1K

Area of Science:

  • Molecular Biology
  • Protein Biochemistry
  • Evolutionary Biology

Background:

  • Protein aggregation is influenced by aggregation-prone regions, often involving beta-strand interactions.
  • Charged residues and prolines are frequently found flanking these regions, reducing aggregation.
  • The evolutionary drivers for this 'aggregation gatekeeper' enrichment remain unclear.

Purpose of the Study:

  • To investigate whether aggregation gatekeepers are structurally determined or functionally selected.
  • To compare the sequence conservation and thermodynamic cost of these residues across protein families.

Main Methods:

  • Analysis of sequence conservation in 2659 protein families from Escherichia coli.
  • Assessment of the thermodynamic cost associated with gatekeeper residues.
  • Comparative analysis of conservation patterns versus structural stability costs.

Main Results:

  • Aggregation gatekeepers exhibit strong selective conservation across diverse protein families.
  • These conserved residues impose a significant thermodynamic cost on protein structure.
  • Conservation patterns are not explained solely by structural constraints.

Conclusions:

  • Aggregation gatekeepers are not merely a consequence of structural constraints.
  • These residues are evolutionarily selected to actively control protein aggregation.
  • This selective pressure highlights a functional role in maintaining protein homeostasis.