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Passive sensitization of human intestinal mast cells
H Nolte1, A Kruse, P Stahl Skov
1Department of Pediatrics, University Hospital of Aarhus, Denmark.
Agents and Actions
|April 1, 1989
Summary
Human intestinal mast cells, isolated using enzymatic dispersion, can be passively sensitized. This method yields functional mast cells with intact Fc-receptors, crucial for IgE binding and immune responses.
Area of Science:
- Gastroenterology
- Immunology
- Cell Biology
Background:
- Human intestinal mast cells play a role in allergic responses.
- Understanding mast cell function requires methods to isolate and study them.
- Passive sensitization is a key mechanism in allergic diseases.
Purpose of the Study:
- To develop a method for dispersing human intestinal mast cells.
- To assess the functional integrity of dispersed mast cells.
- To investigate the capacity of these mast cells for passive sensitization.
Main Methods:
- Mechanical and enzymatic dispersion of human duodenal biopsies.
- Isolation of single mast cell suspensions.
- Passive sensitization using autologous and allogeneic plasma.
Main Results:
- Enzymatic dispersion yielded functional human intestinal mast cells.
- Mast cells demonstrated preserved Fc-receptors, enabling IgE binding.
- Passive sensitization with atopic plasma enhanced mast cell sensitivity and reactivity.
Conclusions:
- Enzymatic dispersion is effective for isolating functional human intestinal mast cells.
- Isolated mast cells retain Fc-receptors for passive sensitization.
- This method provides a tool for studying mast cell-mediated allergic reactions.